Genetic Toxicology Technical Committee (GTTC)

Mission Statement

The mission of this committee is to improve the scientific basis of the interpretation of results from genetic toxicology tests for purposes of more accurate hazard identification and assessment of human risk; to develop follow-up strategies for determining the relevance of test results to human health; to provide a framework for integration of testing results into a risk-based assessment of the effects of chemical exposures on human health; to promote the integration and use of new techniques and scientific knowledge in the evaluation of genetic toxicology; and to monitor and promote the development of innovative tests and testing strategies.

Working Groups

  • Evaluation of New Compounds: Nanomaterials

    The work group has evaluated the current testing paradigm for genotoxicity assessment of nanomaterials and is publishing the findings and recommendations for modifying the tests as needed. They are currently considering publishing a series of protocols for genetic toxicity testing of products containing nanomaterials.

    Leadership Team:
    Rosalie Elespuru, PhD (US Food and Drug Administration)
    Shareen Doak, PhD (Swansea University)

    HESI Staff:
    Connie Chen, PhD, MPH

  • In Vivo Follow-Up

    This work group was formed in 2016. This working group focused on providing more detailed advice about which in vivo tests to choose to follow-up on in vitro positive results and how to conduct the tests. As a first step, the group compared data for 90 chemicals for the transgenic rodent assay, the in vivo comet assay, and cancer data. This work was published in Mutation Research/Genetic Toxicology and Environmental Mutagenesis in January 2019 in an article titled “A Comparison of Transgenic Rodent Mutation and In Vivo Comet Assay Responses for 91 Chemicals.” Two follow-up areas include (1) providing best practices on the in vivo comet assay and collecting data on substances that are positive (i.e., induce micronuclei [MN]) in vivo and (2) comparing the plasma concentrations at the lowest effective dose (LOED) with the lowest effective concentration (LOEC) for MN induction in vitro, which could shed some light as to whether there is any relationship between LOEC and LOED for MN-inducing compounds.

    Leadership Team:
    Dan Roberts, PhD (Charles River Laboratories)
    David Lovell, PhD (St. George’s University of London)
    Matt LeBaron, PhD (Dow Chemical Company)

    HESI Staff:
    E’Lissa Flores, PhD

  • Mode of Action

    The MOA Work Group established four subteams to develop tubulin binding, topoisomerase II inhibition, aurora kinase inhibition, and reactive oxygen species activation adverse outcome pathways (AOPs). These AOPs will be submitted to the OECD AOPWiki site, a public repository for approved AOPs. Once these four subteams complete their work, the goal will be to merge them with the Clean Sheet Work Group.

    Leadership Team:
    Mirjam Luitjen, PhD (National Institute for Public Health and the Environment, RIVM, The Netherlands)
    Jan van Benthem, PhD (National Institute for Public Health and the Environment, RIVM, The Netherlands)
    Stefan Pfuhler, PhD (Procter & Gamble Company)

    HESI Staff:
    Carolina Morell-Pérez, MS

  • Germ Cells

    Work for this group is centered around establishing/enhancing protocols for conducting genotoxicity assessment of effects to germ cells. After a series of publications in 2019, the group is finalizing a review on the impact of analyzing mutations in fast proliferating tissues at 28+28. This group is also considering future projects and the course of action for 2020–2021.

    Leadership Team:
    Jan van Benthem, PhD (National Institute for Public Health and the Environment, RIVM, The Netherlands)
    Francesco Marchetti, PhD (Health Canada)

    HESI Staff:
    Connie Chen, PhD, MPH

  • Pig-a Assay

    This work group had their Detailed Review Paper (DRP) and a Validation/Retrospective Performance Analysis document accepted by OECD. It is currently drafting a Test Guideline to be reviewed and accepted by OECD in the future.

    Leadership Team:
    Steven Dertinger, PhD (Litron Laboratories)
    Bob Heflich, PhD (US Food and Drug Administration)

    HESI Staff:
    Connie Chen, PhD, MPH

  • Quantitative Analysis

    The work group is evaluating chemical data and enhancing tools for genetic toxicology dose-response modeling. The group has a planned 2020 virtual EMGS workshop scheduled for December 2020. Additionally, the group is planning to present at the International Workshops on Genotoxicity Testing (IWGT) 2021 meeting and is working on publishing guidance for the standard use and regulatory acceptance of using the benchmark dose (BMD) approach with genetic toxicology data.

    Leadership Team:
    George Johnson, PhD (Swansea University)
    Andreas Zeller, PhD (Roche)

    HESI Staff:
    E’Lissa Flores, PhD

  • Clean Sheet Testing Strategy

    This work group published a conceptual framework for a next-generation testing strategy for assessment of genomic damage for risk assessment and decision-making in 2016, and a case study applying those concepts in industrial chemicals was published in 2019. The group is currently finalizing an additional case study looking at a pharmaceutical compound for 2020. Afterward, this work group will merge with the MOA Work Group.

    Leadership Team:
    Mirjam Luitjen, PhD (National Institute for Public Health and the Environment, RIVM, The Netherlands)
    Jan van Benthem, PhD (National Institute for Public Health and the Environment, RIVM, The Netherlands)
    Stefan Pfuhler, PhD (Procter & Gamble Company)

    HESI Staff:
    Carolina Morell-Pérez, MS

  • Error-Corrected Sequencing

    This group is evaluating error-corrected next-generation sequencing (NGS) as an alternative methodology for evaluating in vivo mutagenesis. Over the past year, the group worked to qualify this platform in a second species and other mouse strains and began to work out the details on a technology transfer. Currently, the group is discussing the different workstreams/subgroups that would need to be established to move this project to the next phase, along with collaborations with both the eSTAR and CT-TRACs committees.

    Leadership Team:
    Bob Young, MS (MilliporeSigma)
    Francesco Marchetti, PhD (Health Canada)
    Sheroy Minocherhomji, PhD (Amgen)

    HESI Staff:
    Carolina Morell-Pérez, MS

  • In Vitro

    This newly established work group started this year with the following goals: (1) critically evaluate NAMs for in vitro genotoxicity testing, (2) envision how NAMs could expand current in vitro genetic toxicology testing strategies (e.g., developing a weight-of-evidence approach), and (3) make recommendations for creating an “in vitro only” approach for genetic toxicology testing that would meet the needs of various regulatory decision-makers.

    Leadership Team:
    Giel Hendriks, PhD (Toxys B.V.)
    Stepahnie Smith-Roe, PhD (National Institute of Environmental Health Sciences, National Toxicology Program)

    HESI Staff:
    Connie Chen, PhD, MPH

HESI Staff

Leadership Team

  • Mirjam Luitjen, PhD

    National Institute for Public Health and the Environment, RIVM, The Netherlands

  • Leon Stankowski, PhD

    Charles River Laboratories

Committee Events

HESI Sessions at EMGS 2021 Virtual Annual Meeting

Virtual annual meeting, hosted by the Environmental Mutagenesis and Genomics Society (EMGS)

The Botanical Safety Consortium (BSC), Genetic Toxicology Technical Committee (GTTC), and Emerging Systems Toxicology for the Assessment of Risk (eSTAR) Committee will present their work at the Environmental Mutagenesis and Genomics Society (EMGS) 2021 Virtual Annual Meeting on September 15-25, 2021.

Read more

HESI GTTC Committee 2021 Annual Meeting

Virtual meeting, hosted by the HESI GTTC Committee

The HESI GTTC Committee 2021 Annual Meeting will take place on June 1-3, 2021 from 9am-1pm EST. The virtual meeting is open to GTTC Committee participants only.

Read more

GTTC Quantitative Analysis Working Group Workshop

Virtual workshop, hosted by the HESI GTTC Committee

This workshop is a pre-GTTC Annual Meeting workshop to provide extra time to discuss 2020-21 Quantitative Analysis Working Group activities, as well as emerging challenges and upcoming milestones. The presentations will collectively cover a variety of topics and issues pertaining to the refinement of quantitative methods for ...

Read more

Quantitative Interpretation of Genetic Toxicity Dose Response Data for Risk Assessment and Regulatory Decision-Making: Current Status, Persistent Challenges, and Emerging Priorities

Virtual workshop, hosted by HESI and the Environmental Mutagenesis and Genomics Society (EMGS)

The workshop will (1) provide a brief overview of the state of the science regarding quantitative interpretation of genetic toxicity dose-response data, including a brief overview of the BMD combined covariate approach for potency ranking and MOA investigation, (2) a summary of recent genotoxicity risk assessment case ...

Read more

Validation of New Approaches in Genetic Toxicology

Newark, Delaware, USA

Novel in vitro and in vivo assays have been developed to improve the reliability of tools employed for identification of genotoxic hazard. Several of the new assays can provide insight into mode-of-action (MoA). As emerging technologies generally used for evaluating human hazard and/or risk, these novel ...

Read more

Applied Genetic Toxicity for Regulatory Decision Making: The Road Ahead

Potsdam, Germany

Applied genetic toxicology is undergoing a major paradigm shift towards changing the strategies employed for assessment of chemically-induced genomic damage, evaluation methods of the mechanisms by which that damage translates into adverse effects, and the quantitative methods used to interpret dose-response data. This ...

Read more

WP_Query Object
(
    [query] => Array
        (
            [post_type] => event
            [posts_per_page] => 6
            [type] => 
            [area] => 
            [before] => 2050-01-01
            [after] => 
            [tags] => GTTC
            [showtitle] => false
            [meta_query] => Array
                (
                    [relation] => AND
                    [0] => Array
                        (
                            [key] => _ilsi_date
                            [value] => 2050-01-01
                            [compare] => <=
                        )

                    [1] => Array
                        (
                            [key] => _ilsi_end_date
                            [value] => 2050-01-01
                            [compare] => <=
                        )

                )

            [paged] => 1
            [tax_query] => Array
                (
                    [0] => Array
                        (
                            [taxonomy] => post_tag
                            [field] => name
                            [terms] => GTTC
                        )

                )

            [meta_key] => _ilsi_date
            [orderby] => meta_value
            [order] => DESC
        )

    [query_vars] => Array
        (
            [post_type] => event
            [posts_per_page] => 6
            [type] => 
            [area] => 
            [before] => 2050-01-01
            [after] => 
            [tags] => GTTC
            [showtitle] => false
            [meta_query] => Array
                (
                    [relation] => AND
                    [0] => Array
                        (
                            [key] => _ilsi_date
                            [value] => 2050-01-01
                            [compare] => <=
                        )

                    [1] => Array
                        (
                            [key] => _ilsi_end_date
                            [value] => 2050-01-01
                            [compare] => <=
                        )

                )

            [paged] => 1
            [tax_query] => Array
                (
                    [0] => Array
                        (
                            [taxonomy] => post_tag
                            [field] => name
                            [terms] => GTTC
                        )

                )

            [meta_key] => _ilsi_date
            [orderby] => meta_value
            [order] => DESC
            [error] => 
            [m] => 
            [p] => 0
            [post_parent] => 
            [subpost] => 
            [subpost_id] => 
            [attachment] => 
            [attachment_id] => 0
            [name] => 
            [pagename] => 
            [page_id] => 0
            [second] => 
            [minute] => 
            [hour] => 
            [day] => 0
            [monthnum] => 0
            [year] => 0
            [w] => 0
            [category_name] => 
            [tag] => 
            [cat] => 
            [tag_id] => 802
            [author] => 
            [author_name] => 
            [feed] => 
            [tb] => 
            [meta_value] => 
            [preview] => 
            [s] => 
            [sentence] => 
            [title] => 
            [fields] => 
            [menu_order] => 
            [embed] => 
            [category__in] => Array
                (
                )

            [category__not_in] => Array
                (
                )

            [category__and] => Array
                (
                )

            [post__in] => Array
                (
                )

            [post__not_in] => Array
                (
                )

            [post_name__in] => Array
                (
                )

            [tag__in] => Array
                (
                )

            [tag__not_in] => Array
                (
                )

            [tag__and] => Array
                (
                )

            [tag_slug__in] => Array
                (
                )

            [tag_slug__and] => Array
                (
                )

            [post_parent__in] => Array
                (
                )

            [post_parent__not_in] => Array
                (
                )

            [author__in] => Array
                (
                )

            [author__not_in] => Array
                (
                )

            [suppress_filters] => 
            [ignore_sticky_posts] => 
            [cache_results] => 1
            [update_post_term_cache] => 1
            [lazy_load_term_meta] => 1
            [update_post_meta_cache] => 1
            [nopaging] => 
            [comments_per_page] => 50
            [no_found_rows] => 
        )

    [tax_query] => WP_Tax_Query Object
        (
            [queries] => Array
                (
                    [0] => Array
                        (
                            [taxonomy] => post_tag
                            [terms] => Array
                                (
                                    [0] => GTTC
                                )

                            [field] => name
                            [operator] => IN
                            [include_children] => 1
                        )

                )

            [relation] => AND
            [table_aliases:protected] => Array
                (
                    [0] => wp_term_relationships
                )

            [queried_terms] => Array
                (
                    [post_tag] => Array
                        (
                            [terms] => Array
                                (
                                    [0] => GTTC
                                )

                            [field] => name
                        )

                )

            [primary_table] => wp_posts
            [primary_id_column] => ID
        )

    [meta_query] => WP_Meta_Query Object
        (
            [queries] => Array
                (
                    [0] => Array
                        (
                            [key] => _ilsi_date
                        )

                    [1] => Array
                        (
                            [0] => Array
                                (
                                    [key] => _ilsi_date
                                    [value] => 2050-01-01
                                    [compare] => <=
                                )

                            [1] => Array
                                (
                                    [key] => _ilsi_end_date
                                    [value] => 2050-01-01
                                    [compare] => <=
                                )

                            [relation] => AND
                        )

                    [relation] => AND
                )

            [relation] => AND
            [meta_table] => wp_postmeta
            [meta_id_column] => post_id
            [primary_table] => wp_posts
            [primary_id_column] => ID
            [table_aliases:protected] => Array
                (
                    [0] => wp_postmeta
                    [1] => mt1
                    [2] => mt2
                )

            [clauses:protected] => Array
                (
                    [wp_postmeta] => Array
                        (
                            [key] => _ilsi_date
                            [compare] => =
                            [compare_key] => =
                            [alias] => wp_postmeta
                            [cast] => CHAR
                        )

                    [mt1] => Array
                        (
                            [key] => _ilsi_date
                            [value] => 2050-01-01
                            [compare] => <=
                            [compare_key] => =
                            [alias] => mt1
                            [cast] => CHAR
                        )

                    [mt2] => Array
                        (
                            [key] => _ilsi_end_date
                            [value] => 2050-01-01
                            [compare] => <=
                            [compare_key] => =
                            [alias] => mt2
                            [cast] => CHAR
                        )

                )

            [has_or_relation:protected] => 
        )

    [date_query] => 
    [request] => SELECT SQL_CALC_FOUND_ROWS  wp_posts.ID FROM wp_posts  LEFT JOIN wp_term_relationships ON (wp_posts.ID = wp_term_relationships.object_id) INNER JOIN wp_postmeta ON ( wp_posts.ID = wp_postmeta.post_id )  INNER JOIN wp_postmeta AS mt1 ON ( wp_posts.ID = mt1.post_id )  INNER JOIN wp_postmeta AS mt2 ON ( wp_posts.ID = mt2.post_id ) WHERE 1=1  AND ( 
  wp_term_relationships.term_taxonomy_id IN (802)
) AND ( 
  wp_postmeta.meta_key = '_ilsi_date' 
  AND 
  ( 
    ( mt1.meta_key = '_ilsi_date' AND mt1.meta_value <= '2050-01-01' ) 
    AND 
    ( mt2.meta_key = '_ilsi_end_date' AND mt2.meta_value <= '2050-01-01' )
  )
) AND wp_posts.post_type = 'event' AND (wp_posts.post_status = 'publish' OR wp_posts.post_status = 'acf-disabled') GROUP BY wp_posts.ID ORDER BY wp_postmeta.meta_value DESC LIMIT 0, 6
    [posts] => Array
        (
            [0] => WP_Post Object
                (
                    [ID] => 24152
                    [post_author] => 14
                    [post_date] => 2021-09-03 14:41:03
                    [post_date_gmt] => 2021-09-03 14:41:03
                    [post_content] => 
                    [post_title] => HESI Sessions at EMGS 2021 Virtual Annual Meeting
                    [post_excerpt] => 
                    [post_status] => publish
                    [comment_status] => closed
                    [ping_status] => closed
                    [post_password] => 
                    [post_name] => hesi-sessions-at-emgs-2021-virtual-annual-meeting
                    [to_ping] => 
                    [pinged] => 
                    [post_modified] => 2021-09-03 15:22:09
                    [post_modified_gmt] => 2021-09-03 15:22:09
                    [post_content_filtered] => 
                    [post_parent] => 0
                    [guid] => https://hesiglobal.org/?post_type=event&p=24152
                    [menu_order] => 0
                    [post_type] => event
                    [post_mime_type] => 
                    [comment_count] => 0
                    [filter] => raw
                )

            [1] => WP_Post Object
                (
                    [ID] => 23962
                    [post_author] => 14
                    [post_date] => 2021-03-31 18:21:01
                    [post_date_gmt] => 2021-03-31 18:21:01
                    [post_content] => 
                    [post_title] => HESI GTTC Committee 2021 Annual Meeting
                    [post_excerpt] => 
                    [post_status] => publish
                    [comment_status] => closed
                    [ping_status] => closed
                    [post_password] => 
                    [post_name] => hesi-gttc-committee-2021-annual-meeting
                    [to_ping] => 
                    [pinged] => 
                    [post_modified] => 2021-03-31 18:24:10
                    [post_modified_gmt] => 2021-03-31 18:24:10
                    [post_content_filtered] => 
                    [post_parent] => 0
                    [guid] => https://hesiglobal.org/?post_type=event&p=23962
                    [menu_order] => 0
                    [post_type] => event
                    [post_mime_type] => 
                    [comment_count] => 0
                    [filter] => raw
                )

            [2] => WP_Post Object
                (
                    [ID] => 23979
                    [post_author] => 14
                    [post_date] => 2021-04-08 17:48:37
                    [post_date_gmt] => 2021-04-08 17:48:37
                    [post_content] => 
                    [post_title] => GTTC Quantitative Analysis Working Group Workshop
                    [post_excerpt] => 
                    [post_status] => publish
                    [comment_status] => closed
                    [ping_status] => closed
                    [post_password] => 
                    [post_name] => gttc-quantitative-analysis-working-group-workshop
                    [to_ping] => 
                    [pinged] => 
                    [post_modified] => 2021-04-29 14:02:39
                    [post_modified_gmt] => 2021-04-29 14:02:39
                    [post_content_filtered] => 
                    [post_parent] => 0
                    [guid] => https://hesiglobal.org/?post_type=event&p=23979
                    [menu_order] => 0
                    [post_type] => event
                    [post_mime_type] => 
                    [comment_count] => 0
                    [filter] => raw
                )

            [3] => WP_Post Object
                (
                    [ID] => 23708
                    [post_author] => 14
                    [post_date] => 2020-10-27 17:55:16
                    [post_date_gmt] => 2020-10-27 17:55:16
                    [post_content] => 
                    [post_title] => Quantitative Interpretation of Genetic Toxicity Dose Response Data for Risk Assessment and Regulatory Decision-Making: Current Status, Persistent Challenges, and Emerging Priorities
                    [post_excerpt] => 
                    [post_status] => publish
                    [comment_status] => closed
                    [ping_status] => closed
                    [post_password] => 
                    [post_name] => quantitative-interpretation-of-genetic-toxicity-dose-response-data-for-risk-assessment-and-regulatory-decision-making-current-status-persistent-challenges-and-emerging-priorities
                    [to_ping] => 
                    [pinged] => 
                    [post_modified] => 2020-10-30 15:26:58
                    [post_modified_gmt] => 2020-10-30 15:26:58
                    [post_content_filtered] => 
                    [post_parent] => 0
                    [guid] => https://hesiglobal.org/?post_type=event&p=23708
                    [menu_order] => 0
                    [post_type] => event
                    [post_mime_type] => 
                    [comment_count] => 0
                    [filter] => raw
                )

            [4] => WP_Post Object
                (
                    [ID] => 21067
                    [post_author] => 12
                    [post_date] => 2019-03-26 09:22:27
                    [post_date_gmt] => 2019-03-26 14:22:27
                    [post_content] => 
                    [post_title] => Validation of New Approaches in Genetic Toxicology
                    [post_excerpt] => 
                    [post_status] => publish
                    [comment_status] => closed
                    [ping_status] => closed
                    [post_password] => 
                    [post_name] => validation-of-new-approaches-in-genetic-toxicology
                    [to_ping] => 
                    [pinged] => 
                    [post_modified] => 2020-10-28 14:42:06
                    [post_modified_gmt] => 2020-10-28 14:42:06
                    [post_content_filtered] => 
                    [post_parent] => 0
                    [guid] => http://hesiglobal.org/?post_type=event&p=21067
                    [menu_order] => 0
                    [post_type] => event
                    [post_mime_type] => 
                    [comment_count] => 0
                    [filter] => raw
                )

            [5] => WP_Post Object
                (
                    [ID] => 6968
                    [post_author] => 12
                    [post_date] => 2018-05-18 09:10:09
                    [post_date_gmt] => 2018-05-18 14:10:09
                    [post_content] => 
		

Workshop Agenda

Workshop Objectives

Mirjam Luitjen, RIVM

Path Forward for Genetic Toxicity Assessment

Kerry Dearfield, Former USDA

The New Strategy in Practice: Case Study Examples

Veronique Thybaud, Sanofi & Nicholas Ball, DOW

Incorporating Mode of Action into Genetic Toxicity Assessment

Roland Frötschl, BfArM, Germany

Novel Approaches and Technologies to Assess Genotoxic Modes of Action

Stefan Pfuhler, Procter & Gamble

Genetic Toxicity Mini-Adverse Outcome Pathways (mini-AOPs)

Azeddine Elhajouji, Novartis

Approaches for Analyses and Interpretation of Genetic Toxicity Dose-Response

Paul White, Health Canada

Quantitative Dose-Response Analyses for Risk Assessment and Regulatory Decision Making: Issues, Applications, and Challenges

George Johnson, Swansea University

Background and Objectives
Applied genetic toxicology is undergoing a major paradigm shift towards changing the strategies employed for assessment of chemically-induced genomic damage, evaluation methods of the mechanisms by which that damage translates into adverse effects, and the quantitative methods used to interpret dose-response data. This shift entails a movement away from simple dichotomous evaluations of genotoxicity (i.e., yes/no), that only supports identification of potential carcinogens, and towards a greater understanding of the diversity of adverse outcomes related to genomic damage, of the multitude of mechanisms (or modes of action) underscoring genomic damage, and the ability to determine point of departure metrics for human health risk assessment and regulatory decision making. Moreover, advanced technologies to investigate genotoxic mechanisms and to analyze dose-response functions are being developed and incorporated into assessments of genomic damage.

 

This workshop was organized by the HESI Genetic Toxicology Technical Committee (GTTC). The HESI GTTC brings together an international cohort of genetic toxicologists from industry, academia, and government to address issues related to all aspects of genetic toxicity assessment. These aspects include the development and validation of assessment approaches, technologies and strategies for mode-of-action determination, and approaches for data interpretation. The GTTC is comprised of experts in the fields of genetic and general toxicology, risk assessment, and computational biology. The workshop will examine a new generation of testing strategy for assessment of genomic damage, new approaches and technologies for mode-of-action determination and interpretation, and recent developments in quantitative interpretation of genetic toxicity dose-response data.

[post_title] => Applied Genetic Toxicity for Regulatory Decision Making: The Road Ahead [post_excerpt] => [post_status] => publish [comment_status] => closed [ping_status] => closed [post_password] => [post_name] => applied-genetic-toxicity-for-regulatory-decision-making-the-road-ahead [to_ping] => [pinged] => [post_modified] => 2020-10-28 14:43:24 [post_modified_gmt] => 2020-10-28 14:43:24 [post_content_filtered] => [post_parent] => 0 [guid] => http://hesiglobal.org/?post_type=event&p=6968 [menu_order] => 0 [post_type] => event [post_mime_type] => [comment_count] => 0 [filter] => raw ) ) [post_count] => 6 [current_post] => -1 [in_the_loop] => [post] => WP_Post Object ( [ID] => 24152 [post_author] => 14 [post_date] => 2021-09-03 14:41:03 [post_date_gmt] => 2021-09-03 14:41:03 [post_content] => [post_title] => HESI Sessions at EMGS 2021 Virtual Annual Meeting [post_excerpt] => [post_status] => publish [comment_status] => closed [ping_status] => closed [post_password] => [post_name] => hesi-sessions-at-emgs-2021-virtual-annual-meeting [to_ping] => [pinged] => [post_modified] => 2021-09-03 15:22:09 [post_modified_gmt] => 2021-09-03 15:22:09 [post_content_filtered] => [post_parent] => 0 [guid] => https://hesiglobal.org/?post_type=event&p=24152 [menu_order] => 0 [post_type] => event [post_mime_type] => [comment_count] => 0 [filter] => raw ) [comment_count] => 0 [current_comment] => -1 [found_posts] => 14 [max_num_pages] => 3 [max_num_comment_pages] => 0 [is_single] => [is_preview] => [is_page] => [is_archive] => 1 [is_date] => [is_year] => [is_month] => [is_day] => [is_time] => [is_author] => [is_category] => [is_tag] => 1 [is_tax] => [is_search] => [is_feed] => [is_comment_feed] => [is_trackback] => [is_home] => [is_privacy_policy] => [is_404] => [is_embed] => [is_paged] => [is_admin] => [is_attachment] => [is_singular] => [is_robots] => [is_favicon] => [is_posts_page] => [is_post_type_archive] => [query_vars_hash:WP_Query:private] => 4aeccc4d0a6d1cf9747d9708d8908fe8 [query_vars_changed:WP_Query:private] => 1 [thumbnails_cached] => [stopwords:WP_Query:private] => [compat_fields:WP_Query:private] => Array ( [0] => query_vars_hash [1] => query_vars_changed ) [compat_methods:WP_Query:private] => Array ( [0] => init_query_flags [1] => parse_tax_query ) )

Committee Publications

Filter

Permitted Daily Exposure Limits for Noteworthy N-Nitrosamines

A genotoxic carcinogen, N-nitrosodimethylamine (NDMA), was detected as a synthesis impurity in some valsartan drugs in 2018, and other N-nitrosamines, such as N-nitrosodiethylamine (NDEA), were later detected in other sartan products. N-nitrosamines are pro-mutagens that can react with DNA following metabolism to produce ...

Read more

Recommendations for Conducting the Rodent Erythrocyte Pig‐a Assay: A Report from the HESI GTTC Pig‐a Workgroup

The rodent Pig‐a assay is a flow cytometric, phenotype‐based method used to measure in vivo somatic cell mutation. An Organization for Economic Co‐operation and Development (OECD) test guideline is currently being developed to support routine use of the assay for regulatory purposes (OECD project number ...

Read more

A Comparison of Transgenic Rodent Mutation and In Vivo Comet Assay Responses for 91 Chemicals

Whilst the TGR and comet assays are available for measuring genotoxic effects in multiple rodent tissues, there has been no systematic comparison of their performance in detecting genotoxicity per se. A working group of the Genetic Toxicology Technical Committee (GTTC), part of the Health and Environmental Sciences Institute ...

Read more

Identifying Germ Cell Mutagens Using OECD Test Guideline 488 (Transgenic Rodent Somatic and Germ Cell Gene Mutation Assays) and Integration with Somatic Cell Testing

The Organisation for Economic Co-operation and Development Test Guideline 488 (TG 488) provides recommendations for assessing germ cell and somatic cell mutagenicity using transgenic rodent (TGR) models. However, important data gaps exist for selecting an optimal approach for simultaneously evaluating mutagenicity in ...

Read more

Simulation of Mouse and Rat Spermatogenesis to Inform Genotoxicity Testing Using OECD Test Guideline 488

The Germ Cell Workgroup of the Genetic Toxicology (GTTC) Committee of the Health and Environmental Sciences Institute modeled mouse and rat spermatogenesis to better define the exposure history of the cell population collected from seminiferous tubules.

Read more

WP_Query Object
(
    [query] => Array
        (
            [post_type] => publication
            [posts_per_page] => 6
            [type] => 
            [area] => 
            [committee] => GTTC
            [authors] => true
            [showtitle] => false
            [meta_query] => Array
                (
                    [relation] => AND
                )

            [tax_query] => Array
                (
                    [0] => Array
                        (
                            [taxonomy] => ilsi_committee
                            [field] => name
                            [terms] => GTTC
                        )

                )

            [paged] => 1
            [meta_key] => _ilsi_date
            [orderby] => meta_value
            [order] => DESC
        )

    [query_vars] => Array
        (
            [post_type] => publication
            [posts_per_page] => 6
            [type] => 
            [area] => 
            [committee] => GTTC
            [authors] => true
            [showtitle] => false
            [meta_query] => Array
                (
                    [relation] => AND
                )

            [tax_query] => Array
                (
                    [0] => Array
                        (
                            [taxonomy] => ilsi_committee
                            [field] => name
                            [terms] => GTTC
                        )

                )

            [paged] => 1
            [meta_key] => _ilsi_date
            [orderby] => meta_value
            [order] => DESC
            [error] => 
            [m] => 
            [p] => 0
            [post_parent] => 
            [subpost] => 
            [subpost_id] => 
            [attachment] => 
            [attachment_id] => 0
            [name] => 
            [pagename] => 
            [page_id] => 0
            [second] => 
            [minute] => 
            [hour] => 
            [day] => 0
            [monthnum] => 0
            [year] => 0
            [w] => 0
            [category_name] => 
            [tag] => 
            [cat] => 
            [tag_id] => 
            [author] => 
            [author_name] => 
            [feed] => 
            [tb] => 
            [meta_value] => 
            [preview] => 
            [s] => 
            [sentence] => 
            [title] => 
            [fields] => 
            [menu_order] => 
            [embed] => 
            [category__in] => Array
                (
                )

            [category__not_in] => Array
                (
                )

            [category__and] => Array
                (
                )

            [post__in] => Array
                (
                )

            [post__not_in] => Array
                (
                )

            [post_name__in] => Array
                (
                )

            [tag__in] => Array
                (
                )

            [tag__not_in] => Array
                (
                )

            [tag__and] => Array
                (
                )

            [tag_slug__in] => Array
                (
                )

            [tag_slug__and] => Array
                (
                )

            [post_parent__in] => Array
                (
                )

            [post_parent__not_in] => Array
                (
                )

            [author__in] => Array
                (
                )

            [author__not_in] => Array
                (
                )

            [suppress_filters] => 
            [ignore_sticky_posts] => 
            [cache_results] => 1
            [update_post_term_cache] => 1
            [lazy_load_term_meta] => 1
            [update_post_meta_cache] => 1
            [nopaging] => 
            [comments_per_page] => 50
            [no_found_rows] => 
            [taxonomy] => ilsi_committee
            [term_id] => GTTC
        )

    [tax_query] => WP_Tax_Query Object
        (
            [queries] => Array
                (
                    [0] => Array
                        (
                            [taxonomy] => ilsi_committee
                            [terms] => Array
                                (
                                    [0] => GTTC
                                )

                            [field] => name
                            [operator] => IN
                            [include_children] => 1
                        )

                )

            [relation] => AND
            [table_aliases:protected] => Array
                (
                    [0] => wp_term_relationships
                )

            [queried_terms] => Array
                (
                    [ilsi_committee] => Array
                        (
                            [terms] => Array
                                (
                                    [0] => GTTC
                                )

                            [field] => name
                        )

                )

            [primary_table] => wp_posts
            [primary_id_column] => ID
        )

    [meta_query] => WP_Meta_Query Object
        (
            [queries] => Array
                (
                    [0] => Array
                        (
                            [key] => _ilsi_date
                        )

                    [relation] => OR
                )

            [relation] => AND
            [meta_table] => wp_postmeta
            [meta_id_column] => post_id
            [primary_table] => wp_posts
            [primary_id_column] => ID
            [table_aliases:protected] => Array
                (
                    [0] => wp_postmeta
                )

            [clauses:protected] => Array
                (
                    [wp_postmeta] => Array
                        (
                            [key] => _ilsi_date
                            [compare] => =
                            [compare_key] => =
                            [alias] => wp_postmeta
                            [cast] => CHAR
                        )

                )

            [has_or_relation:protected] => 
        )

    [date_query] => 
    [request] => SELECT SQL_CALC_FOUND_ROWS  wp_posts.ID FROM wp_posts  LEFT JOIN wp_term_relationships ON (wp_posts.ID = wp_term_relationships.object_id) INNER JOIN wp_postmeta ON ( wp_posts.ID = wp_postmeta.post_id ) WHERE 1=1  AND ( 
  wp_term_relationships.term_taxonomy_id IN (77)
) AND ( 
  wp_postmeta.meta_key = '_ilsi_date'
) AND wp_posts.post_type = 'publication' AND (wp_posts.post_status = 'publish' OR wp_posts.post_status = 'acf-disabled') GROUP BY wp_posts.ID ORDER BY wp_postmeta.meta_value DESC LIMIT 0, 6
    [posts] => Array
        (
            [0] => WP_Post Object
                (
                    [ID] => 24047
                    [post_author] => 14
                    [post_date] => 2021-06-08 14:22:29
                    [post_date_gmt] => 2021-06-08 14:22:29
                    [post_content] => 
                    [post_title] => Permitted Daily Exposure Limits for Noteworthy N-Nitrosamines
                    [post_excerpt] => 
                    [post_status] => publish
                    [comment_status] => closed
                    [ping_status] => closed
                    [post_password] => 
                    [post_name] => permitted-daily-exposure-limits-for-noteworthy-n-nitrosamines
                    [to_ping] => 
                    [pinged] => 
                    [post_modified] => 2021-06-08 14:22:29
                    [post_modified_gmt] => 2021-06-08 14:22:29
                    [post_content_filtered] => 
                    [post_parent] => 0
                    [guid] => https://hesiglobal.org/?post_type=publication&p=24047
                    [menu_order] => 0
                    [post_type] => publication
                    [post_mime_type] => 
                    [comment_count] => 0
                    [filter] => raw
                )

            [1] => WP_Post Object
                (
                    [ID] => 23901
                    [post_author] => 14
                    [post_date] => 2021-03-05 15:24:51
                    [post_date_gmt] => 2021-03-05 15:24:51
                    [post_content] => 
                    [post_title] => Recommendations for Conducting the Rodent Erythrocyte Pig‐a Assay: A Report from the HESI GTTC Pig‐a Workgroup
                    [post_excerpt] => 
                    [post_status] => publish
                    [comment_status] => closed
                    [ping_status] => closed
                    [post_password] => 
                    [post_name] => recommendations-for-conducting-the-rodent-erythrocyte-pig%e2%80%90a-assay-a-report-from-the-hesi-gttc-pig%e2%80%90a-workgroup
                    [to_ping] => 
                    [pinged] => 
                    [post_modified] => 2021-03-05 18:12:50
                    [post_modified_gmt] => 2021-03-05 18:12:50
                    [post_content_filtered] => 
                    [post_parent] => 0
                    [guid] => https://hesiglobal.org/?post_type=publication&p=23901
                    [menu_order] => 0
                    [post_type] => publication
                    [post_mime_type] => 
                    [comment_count] => 0
                    [filter] => raw
                )

            [2] => WP_Post Object
                (
                    [ID] => 21725
                    [post_author] => 14
                    [post_date] => 2019-09-06 12:50:25
                    [post_date_gmt] => 2019-09-06 17:50:25
                    [post_content] => 
                    [post_title] => A Comparison of Transgenic Rodent Mutation and In Vivo Comet Assay Responses for 91 Chemicals
                    [post_excerpt] => 
                    [post_status] => publish
                    [comment_status] => closed
                    [ping_status] => closed
                    [post_password] => 
                    [post_name] => a-comparison-of-transgenic-rodent-mutation-and-in-vivo-comet-assay-responses-for-91-chemicals
                    [to_ping] => 
                    [pinged] => 
                    [post_modified] => 2020-10-28 14:48:16
                    [post_modified_gmt] => 2020-10-28 14:48:16
                    [post_content_filtered] => 
                    [post_parent] => 0
                    [guid] => https://hesiglobal.org/?post_type=publication&p=21725
                    [menu_order] => 0
                    [post_type] => publication
                    [post_mime_type] => 
                    [comment_count] => 0
                    [filter] => raw
                )

            [3] => WP_Post Object
                (
                    [ID] => 21724
                    [post_author] => 14
                    [post_date] => 2019-09-06 12:43:14
                    [post_date_gmt] => 2019-09-06 17:43:14
                    [post_content] => 
                    [post_title] => Analysis of Historical Negative Control Group Data from the In Vitro Micronucleus Assay Using Human Lymphocytes
                    [post_excerpt] => 
                    [post_status] => publish
                    [comment_status] => closed
                    [ping_status] => closed
                    [post_password] => 
                    [post_name] => analysis-of-historical-negative-control-group-data-from-the-in-vitro-micronucleus-assay-using-human-lymphocytes
                    [to_ping] => 
                    [pinged] => 
                    [post_modified] => 2020-10-28 14:49:49
                    [post_modified_gmt] => 2020-10-28 14:49:49
                    [post_content_filtered] => 
                    [post_parent] => 0
                    [guid] => https://hesiglobal.org/?post_type=publication&p=21724
                    [menu_order] => 0
                    [post_type] => publication
                    [post_mime_type] => 
                    [comment_count] => 0
                    [filter] => raw
                )

            [4] => WP_Post Object
                (
                    [ID] => 21726
                    [post_author] => 14
                    [post_date] => 2019-09-06 12:55:31
                    [post_date_gmt] => 2019-09-06 17:55:31
                    [post_content] => 
                    [post_title] => Identifying Germ Cell Mutagens Using OECD Test Guideline 488 (Transgenic Rodent Somatic and Germ Cell Gene Mutation Assays) and Integration with Somatic Cell Testing
                    [post_excerpt] => 
                    [post_status] => publish
                    [comment_status] => closed
                    [ping_status] => closed
                    [post_password] => 
                    [post_name] => identifying-germ-cell-mutagens-using-oecd-test-guideline-488-transgenic-rodent-somatic-and-germ-cell-gene-mutation-assays-and-integration-with-somatic-cell-testing
                    [to_ping] => 
                    [pinged] => 
                    [post_modified] => 2020-10-28 14:50:40
                    [post_modified_gmt] => 2020-10-28 14:50:40
                    [post_content_filtered] => 
                    [post_parent] => 0
                    [guid] => https://hesiglobal.org/?post_type=publication&p=21726
                    [menu_order] => 0
                    [post_type] => publication
                    [post_mime_type] => 
                    [comment_count] => 0
                    [filter] => raw
                )

            [5] => WP_Post Object
                (
                    [ID] => 7285
                    [post_author] => 12
                    [post_date] => 2018-08-14 09:26:22
                    [post_date_gmt] => 2018-08-14 14:26:22
                    [post_content] => 
                    [post_title] => Simulation of Mouse and Rat Spermatogenesis to Inform Genotoxicity Testing Using OECD Test Guideline 488
                    [post_excerpt] => 
                    [post_status] => publish
                    [comment_status] => closed
                    [ping_status] => closed
                    [post_password] => 
                    [post_name] => simulation-of-mouse-and-rat-spermatogenesis-to-inform-genotoxicity-testingusing-oecd-test-guideline-488
                    [to_ping] => 
                    [pinged] => 
                    [post_modified] => 2020-10-28 14:52:06
                    [post_modified_gmt] => 2020-10-28 14:52:06
                    [post_content_filtered] => 
                    [post_parent] => 0
                    [guid] => http://hesiglobal.org/?post_type=publication&p=7285
                    [menu_order] => 0
                    [post_type] => publication
                    [post_mime_type] => 
                    [comment_count] => 0
                    [filter] => raw
                )

        )

    [post_count] => 6
    [current_post] => -1
    [in_the_loop] => 
    [post] => WP_Post Object
        (
            [ID] => 24047
            [post_author] => 14
            [post_date] => 2021-06-08 14:22:29
            [post_date_gmt] => 2021-06-08 14:22:29
            [post_content] => 
            [post_title] => Permitted Daily Exposure Limits for Noteworthy N-Nitrosamines
            [post_excerpt] => 
            [post_status] => publish
            [comment_status] => closed
            [ping_status] => closed
            [post_password] => 
            [post_name] => permitted-daily-exposure-limits-for-noteworthy-n-nitrosamines
            [to_ping] => 
            [pinged] => 
            [post_modified] => 2021-06-08 14:22:29
            [post_modified_gmt] => 2021-06-08 14:22:29
            [post_content_filtered] => 
            [post_parent] => 0
            [guid] => https://hesiglobal.org/?post_type=publication&p=24047
            [menu_order] => 0
            [post_type] => publication
            [post_mime_type] => 
            [comment_count] => 0
            [filter] => raw
        )

    [comment_count] => 0
    [current_comment] => -1
    [found_posts] => 23
    [max_num_pages] => 4
    [max_num_comment_pages] => 0
    [is_single] => 
    [is_preview] => 
    [is_page] => 
    [is_archive] => 1
    [is_date] => 
    [is_year] => 
    [is_month] => 
    [is_day] => 
    [is_time] => 
    [is_author] => 
    [is_category] => 
    [is_tag] => 
    [is_tax] => 1
    [is_search] => 
    [is_feed] => 
    [is_comment_feed] => 
    [is_trackback] => 
    [is_home] => 
    [is_privacy_policy] => 
    [is_404] => 
    [is_embed] => 
    [is_paged] => 
    [is_admin] => 
    [is_attachment] => 
    [is_singular] => 
    [is_robots] => 
    [is_favicon] => 
    [is_posts_page] => 
    [is_post_type_archive] => 
    [query_vars_hash:WP_Query:private] => aaf30fc37dbe407e18dcfd454baa2a10
    [query_vars_changed:WP_Query:private] => 1
    [thumbnails_cached] => 
    [stopwords:WP_Query:private] => 
    [compat_fields:WP_Query:private] => Array
        (
            [0] => query_vars_hash
            [1] => query_vars_changed
        )

    [compat_methods:WP_Query:private] => Array
        (
            [0] => init_query_flags
            [1] => parse_tax_query
        )

)

Contact Us

Health and Environmental Sciences Institute (HESI)

hesi@hesiglobal.org
Phone: +1-202-659-8404
Fax: +1-202-659-3859

740 15th Street NW, Suite 600
Washington, DC 20005

Stay Informed

Sign up for our monthly e-newsletter.