Protease resistance of food proteins: a mixed picture for predicting allergenicity but a useful tool for assessing exposure


Background: Susceptibility to pepsin digestion of candidate transgene products is regarded an important param‑ eter in the weight-of-evidence approach for allergenicity risk assessment of genetically modifed crops. It has been argued that protocols used for this assessment should better refect physiological conditions encountered in repre‑ sentative food consumption scenarios.
Aim: To evaluate whether inclusion of more physiological conditions, such as sub-optimal and lower pepsin con‑ centrations, in combination with pancreatin digestion, improved the performance of digestibility protocols used in characterization of protein stability.
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