Benchmark Response Values for Error-Corrected Sequencing Mutagenicity Assessment Technologies

HESI Global’s Genetic Toxicology Technical Committee is pleased to share a new publication in Environmental and Molecular Mutagenesis: “Benchmark Response Values for Error-Corrected Sequencing Mutagenicity Assessment Technologies.”

The paper advanced mutagenicity risk assessment with standardized benchmark response (BMR) for error-corrected sequencing (ECS) technologies.

Key Points:
· Introduces evidence-BMR values tailored to advanced ECS technologies used in mutagenicity testing. 
· Finds consistent variability across experimental conditions, supporting use of standardized, technology-specific BMRs. 
· Recommends a ~30% BMR as a robust default for in vivo ECS-based mutagenicity assessments. 
· Enhances confidence in dose–response modeling and strengthens applications in human health risk assessment and regulatory decision-making. 
· Supports integration of next-generation sequencing tools (e.g., Duplex Sequencing, Hawk-Seq) into quantitative toxicology frameworks.

Figure 2 (shown right) shows the BMD analysis of 47 DupSeq datasets.

Read the full publication here: Mulugeta et al., 2026. Benchmark Response Values for Error-Corrected Sequencing Mutagenicity Assessment Technologies. Environmental and Molecular Mutagenesis. https://doi.org/10.1002/em.70051

Learn more about HESI Global’s GTTC here: https://hesiglobal.org/genetic-toxicology-gttc/