A multi-detector analytical approach for characterizing complex botanical extracts: a case study on ashwagandha

Ensuring the safety and authenticity of botanical supplements starts with understanding what’s inside. In a new case study on ashwagandha, the HESI Global Botanical Safety Consortium showcases a multi-detector analytical platform designed to do just that.

This innovative approach combines *UHPLC, PDA, CAD, and HRMS to:

✔️Identify and semi-quantify over 60 constituents
✔️Support material authentication and detect variability
✔️Enable in silico and in vitro safety evaluations

By generating detailed chemical fingerprints, this method helps advance consistent, science-based assessments of complex botanical products—contributing to both consumer confidence and product innovation.

Read the publication:
Sica, V.P., Mitchell, C.A., Krzykwa, J. et al. A multi-detector analytical approach for characterizing complex botanical extracts: a case study on ashwagandha. Anal Bioanal Chem (2025). https://doi.org/10.1007/s00216-025-06006-8

Learn more about the Botanical Safety Consortium: botanicalsafetyconsortium.org

* Ultra-high-performance liquid chromatography (UHPLC) coupled with photodiode array (PDA) detection, charged aerosol detection (CAD), and high-resolution mass spectrometry (HRMS)