Protein Allergenicity Technical Committee (PATC)

 

Protein Allergenicity Technical Committee (PATC)

Mission Statement

An important component of the safety assessment of biotechnology products is to make a determination of the allergenic potential of newly expressed proteins. The mission of the PATC is to advance the scientific understanding of relevant parameters for characterizing the allergenic potential of novel proteins and biotechnology products.

Committee Contacts

HESI Staff

Lucilia Mouries, PhD
lmouries@hesiglobal.org

 Lauren Peel
lpeel@hesiglobal.org

Leadership Team

Greg Ladics, PhD
E.I. DuPont de Nemours and Company

Scott McClain, PhD
Syngenta

Ronald van Ree, PhD
Academic Medical Center, Amsterdam University

Members and Fact Sheet

Committee Events

Filter

Events From 2030-12-01—2015-01-01

PATC Non-IgE Mediated Immune Reactions to Foods Workshop

Rome, Italy

This workshop provided an opportunity to discuss the current state of the science in immunologic mechanisms of non-IgE mediated food allergy with a specific emphasis on use of these data to assess food safety.

Read more

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Committee Publications

All Publications

Inter-laboratory optimization of protein extraction, separation, and fluorescent detection of endogenous rice allergens

In rice, several allergens have been identified such as the non-specific lipid transfer protein-1, the α-amylase/trypsin-inhibitors, the α-globulin, the 33 kDa glyoxalase I (Gly I), the 52–63 kDa globulin, and the granule-bound starch synthetase. The goal of the present study was to define optimal rice extraction and detection methods that would allow a sensitive and reproducible measure of several classes of known rice allergens. In a three-laboratory ring-trial experiment, several protein extraction methods were first compared and analyzed by 1D multiplexed SDS-PAGE. In a second phase, an inter-laboratory validation of 2D-DIGE analysis was conducted in five independent laboratories, focusing on three rice allergens (52 kDa globulin, 33 kDa glyoxalase I, and 14–16 kDa α-amylase/trypsin inhibitor family members). The results of the present study indicate that a combination of 1D multiplexed SDS-PAGE and 2D-DIGE methods would be recommended to quantify the various rice allergens.

Please click here to view this open access publication.

Read more

Assessment of potential adjuvanticity of Cry proteins

Genetically modified (GM) crops have achieved success in the marketplace and their benefits extend beyond the overall increase in harvest yields to include lowered use of insecticides and decreased carbon dioxide emissions. The most widely grown GM crops contain gene/s for targeted insect protection, herbicide tolerance, or both. Plant expression of Bacillus thuringiensis (Bt) crystal (Cry) insecticidal proteins have been the primary way to impart insect resistance in GM crops. Although deemed safe by regulatory agencies globally, previous studies have been the basis for discussions around the potential immuno-adjuvant effects of Cry proteins. These studies had limitations in study design. The studies used animal models with extremely high doses of Cry proteins, which when given using the ig route were co-administered with an adjuvant. Although the presumption exists that Cry proteins may have immunostimulatory activity and therefore an adjuvanticity risk, the evidence shows that Cry proteins are expressed at very low levels in GM crops and are unlikely to function as adjuvants. This conclusion is based on critical review of the published literature on the effects of immunomodulation by Cry proteins, the history of safe use of Cry proteins in foods, safety of the Bt donor organisms, and pre-market weight-of-evidence-based safety assessments for GM crops.

Read more

Genetic Basis and Detection of Unintended Effects in Genetically Modified Crop Plants

In January 2014, an international meeting sponsored by the International Life Sciences Institute/Health and Environmental Sciences Institute and the Canadian Food Inspection Agency titled “Genetic Basis of Unintended Effects in Modified Plants” was held in Ottawa, Canada, bringing together over 75 scientists from academia, government, and the agro-biotech industry.

Read more

Agricultural Biotechnology Safety Assessment

Genetically modified (GM) crops were first introduced to farmers in 1995 with the intent to provide better crop yield and meet the increasing demand for food and feed.

Read more

Food Allergy: Definitions, Prevalence, Diagnosis and Therapy

Food allergy is phenotypically an extremely heterogeneous group of diseases affecting multiple organs, sometimes in an isolated way, sometimes simultaneously, with the severity of reactions ranging from mild and local to full-blown anaphylaxis.

Read more

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